Article
Structure-function studies of the myosin motor domain: importance of the 50-kDa cleft.
Molecular biology of the cell - 1 Jul 1996
Ruppel K M, Spudich J A
Abstract excerpt
We used random mutagenesis to create 21 point mutations in a highly conserved region of the motor domain of Dictyostelium myosin and classified them into three distinct groups based on the ability to complement myosin null cell phenotypes: wild type, intermediate, and null. Biochemical analysis o...
Topics
- Actins
- Adenosine Triphosphate
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Biomechanical Phenomena
- Chickens
- Dictyostelium
- Genes, Fungal
- Genes, Protozoan
- Models, Molecular
- Molecular Sequence Data
- Molecular Structure
- Mutagenesis
- Myosin Subfragments
- Myosins
- Oligodeoxyribonucleotides