Article
Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium.
EMBO reports - 1 Nov 2002
Tsiavaliaris Georgios, Fujita-Becker Setsuko, Batra Renu, Levitsky Dmitrii I, Kull F Jon, Geeves Michael A, Manstein Dietmar J
Abstract excerpt
Dominant-negative inhibition is a powerful genetic tool for the characterization of gene function in vivo, based on the specific impairment of a gene product by the coexpression of a mutant version of the same gene product. We describe the detailed characterization of two myosin constructs containing either point mutations F487A or F506G in the relay region. Dictyostelium cells transformed with F487A or F506G...
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