Article
Green fluorescent protein expressed by recombinant pseudorabies virus as an in vivo marker for viral replication.
Journal of virological methods - 1 Jul 1997
Jöns A, Mettenleiter T C
Abstract excerpt
We isolated and characterized a pseudorabies virus (PrV) mutant expressing an engineered green fluorescent protein (GFP) optimized for expression in human cells. The GFP DNA was inserted in the non-essential glycoprotein G (gG) gene of the attenuated PrV strain Bartha. The coding sequence was clo...
Topics
- Animals
- Cattle
- Cell Line
- DNA, Viral
- Fluorescent Antibody Technique, Indirect
- Genes, Reporter
- Genetic Markers
- Green Fluorescent Proteins
- Herpesvirus 1, Suid
- Humans
- Kidney
- Luminescent Proteins
- Microscopy, Fluorescence
- Mutation
- Recombinant Fusion Proteins
- Viral Envelope Proteins
- Virus Replication
