Article
Expression of human 21-hydroxylase (P450c21) in bacterial and mammalian cells: a system to characterize normal and mutant enzymes.
Molecular endocrinology (Baltimore, Md.) - 1 Jun 1990
Hu M C, Chung B C
Abstract excerpt
Cytochrome P450c21 (steroid 21-hydroxylase) is a key enzyme in the synthesis of cortisol, whose deficiency is the cause of a common genetic disease, congenital adrenal hyperplasia. We have expressed P450c21 (steroid 21-hydroxylase) in E. coli and mammalian cells. In E. coli, P450c21 cDNA was clon...
Topics
- Adrenal Hyperplasia, Congenital
- Amino Acid Sequence
- Cell Line
- Cytochrome P-450 Enzyme System
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Humans
- Immunoblotting
- Molecular Sequence Data
- Mutation
- Plasmids
- Steroid 21-Hydroxylase
- Transfection