Article
Modeling and mutagenesis of the active site of human P450c17.
Molecular endocrinology (Baltimore, Md.) - 1 Mar 1994
Lin D, Zhang L H, Chiao E, Miller W L
Abstract excerpt
Cytochrome P450c17 is the single enzyme having steroid 17 alpha-hydroxylase and 17,20 lyase activities. We sought to model the active site of this enzyme to identify residues contributing to its catalytic activities, and to test the roles of the identified amino acids by altering them via site-directed mutagenesis. Using the MIDAS-plus program, we modeled P450c17 and the structurally related steroid...
Topics
- Aldehyde-Lyases
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cytochrome P-450 Enzyme System
- DNA
- Genetic Vectors
- Humans
- Image Processing, Computer-Assisted
- Immunoblotting
- Models, Molecular