Article
Expression, purification, and enzymatic properties of recombinant human cytochrome P450c27 (CYP27).
Archives of biochemistry and biophysics - 1 Jul 1997
Pikuleva I A, Björkhem I, Waterman M R
Abstract excerpt
A large number of microsomal P450s have been expressed in Escherichia coli in quantities sufficient for structure/function analysis. However, only one mitochondrial P450 has been successfully overexpressed, that being cholesterol side chain cleavage cytochrome P450 (P450scc). We report here overe...
Topics
- Amino Acid Sequence
- Animals
- Cholestanetriol 26-Monooxygenase
- Cholestanols
- Chromatography, DEAE-Cellulose
- Chromatography, High Pressure Liquid
- Cloning, Molecular
- Cytochrome P-450 Enzyme System
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Humans
- Hydroxylation
- Mutation
- Rabbits
- Rats
- Recombinant Proteins
- Steroid Hydroxylases