Article
The Streptomyces lividans 66 chromosome contains a 1 MB deletogenic region flanked by two amplifiable regions.
Molecular & general genetics : MGG - 1 Nov 1993
Redenbach M, Flett F, Piendl W, Glocker I, Rauland U, Wafzig O, Kliem R, Leblond P, Cullum J
Abstract excerpt
Genetic instability in Streptomyces species often involves large deletions sometimes accompanied by DNA amplification. Two such systems in Streptomyces lividans 66 involve the production of mutants sensitive to chloramphenicol and the production of mutants resistant to the galactose analogue 2-deoxygalactose, respectively. Overlapping cosmids were isolated that span the ca. 1 Mb region between the two amplifiable...
Topics
- Chloramphenicol
- Chromosome Deletion
- Chromosome Walking
- Chromosomes, Bacterial
- Cloning, Molecular
- Drug Resistance, Microbial
- Electrophoresis, Gel, Pulsed-Field
- Fucose
- Gene Amplification
- Mutation