Article
Cloning, sequencing, and expression of a Thermomonospora fusca protease gene in Streptomyces lividans.
Applied and environmental microbiology - 1 Nov 1996
Lao G, Wilson D B
Abstract excerpt
The major Thermomonospora fusca YX extracellular protease gene (tfpA) was cloned into Escherichia coli and Streptomyces lividans and was sequenced. The open reading frame encoded 375 residues, including a 31-residue potential signal sequence, an N-terminal prosequence containing 150 residues, and the 194-residue mature protease that belongs to the chymotrypsin family. The protease was secreted by S. lividans, but...
Topics
- Actinomycetales
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Open Reading Frames
- Serine Endopeptidases
- Streptomyces