Article
Molecular cloning and characterization of a novel glucocerebrosidase of Paenibacillus sp. TS12.
Journal of biochemistry - 1 Aug 2002
Sumida Tomomi, Sueyoshi Noriyuki, Ito Makoto
Abstract excerpt
We report here the molecular cloning and characterization of a glucocerebrosidase [EC 3.2.1.45] from Paenibacillus sp. TS12. The open reading frame of the glucocerebrosidase gene consisted of 2,493 bp nucleotides and encoded 831 amino acid residues. The enzyme exhibited no sequence similarity with a classical glucocerebrosidase belonging to glycoside hydrolase (GH) family 30, but rather showed significant...
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