Article
A strategy for the identification of candidate genes for alcohol-related phenotypes and other human disorders using rapid polymerase chain reaction mapping of gene-based sequence-tagged sites.
Alcoholism, clinical and experimental research - 1 Aug 1995
Berry R, Walter N A, Stevens T J, Rubano T, Wilcox A S, Hopkins J A, Sikela J M
Abstract excerpt
We describe a method for the rapid identification and mapping of human genes, including those possibly contributing to disease and alcohol-related phenotypes. New human genes are identified from cDNA libraries through single-pass sequencing into the 3' untranslated (3'UT) regions of human brain cDNAs. Primers derived from the 3'UT region sequences [representing gene-based, sequence-tagged sites (STSs)] are used...
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