Article
UV-mutagenesis at a cloned target sequence: converted suppressor mutation is insensitive to mutation frequency decline regardless of the gene orientation.
Mutation research - 1 Jan 2000
Engstrom J, Larsen S, Rogers S, Bockrath R
Abstract excerpt
Premutational lesions produced by ultraviolet radiation in the Gln2 tRNA genes of E. coli B/r show differing sensitivities to a mutation avoidance phenomenon known as mutation frequency decline (MFD). A mutation event that changes the wild-type gene to an amber (UAG) suppressor is normally sensitive to MFD. Mutation of this amber suppressor to an ochre (UAA) suppressor is not sensitive to MFD. These two mutation...
Topics
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genes, Bacterial
- Genotype
- Mutation
- Suppression, Genetic
- Ultraviolet Rays