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Reversion of argE3 to Arg(+) in Escherichia coli AB1157 -an informative bacterial system for mutation detection.

Acta biochimica Polonica - 1 Jan 2010

Sikora Anna, Grzesiuk Elżbieta

Abstract excerpt

This review concerns reversion of the argE3 (ochre) nonsense mutation to prototrophy in E. coli AB1157 strain as an informative system for mutation detection. Strain AB1157 bears the argE3 (ochre), hisG4 (ochre) and thr-1 (amber) mutations, and the supE44 amber suppressor on its chromosome. The Arg(+) phenotype can be restored by (i) any base substitution at the argE3 site that changes the nonsense UAA codon to...

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PMID: 20978633

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