Bridging non-overlapping reads illuminates high-order epistasis between distal protein sites in a GPCR.

Abstract excerpt

Epistasis emerges when the effects of an amino acid depend on the identities of interacting residues. This phenomenon shapes fitness landscapes, which have the power to reveal evolutionary paths and inform evolution of desired functions. However, there is a need for easily implemented, high-throughput methods to capture epistasis particularly at distal sites. Here, we combine deep mutational scanning (DMS) with a...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Amino Acid Motifs
• Share to Binding Sites
• Share to Epistasis, Genetic