Cell Painting in activated cells illuminates phenotypic dark space and drug mechanisms of action.

Abstract excerpt

As drug and natural product libraries expand, assays for assessing mechanisms of action (MoA) are increasingly critical. Performing cytological profiling using the Cell Painting (CP) assay enables image-based profiling of cellular states upon treatment, yet many bioactive compounds remain uncharacterized due to undetectable cellular effects under standard conditions. To address this, we combine drug dosing with...

Topics

• Humans
• A549 Cells
• Phenotype
• Protein Kinase C
• Receptors, Glucocorticoid
• Tetradecanoylphorbol Acetate