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Article

Cell Painting PLUS: An Iterative Staining-Elution Protocol for High-Content Phenotypic Screenings.

Current protocols - 1 Apr 2026

Wedler Marlene, von Coburg Elena, Muino Jose M, Valentin Lars, Körber Nils, Dunst Sebastian, Liu Shu

Abstract excerpt

Cell Painting (CP) methods use a combination of fluorescent dyes to label multiple cellular compartments simultaneously, enabling the comprehensive analysis of phenotypic changes through morphological profiling. Here, we present a detailed protocol for the Cell Painting PLUS (CPP) method along with an automated image and data analysis strategy. In CPP, the iterative staining, elution, and re-staining of cells...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 42037520
DOI: 10.1002/cpz1.70368
PMCID: PMC13112130

Genes

CP

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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