Generation of Gene Knock-Out Mutants in Ustilago maydis Using Cas9hf Nuclease.

Abstract excerpt

Gene disruption of nonessential genes became more convenient with the adaptation of the CRISPR-Cas9 system for Ustilago maydis by the group of Regine Kahmann (MPI Marburg, Germany) in 2016. In our group, we have developed the system further to create defined marker-free gene deletions. Therefore, we used the CRISPR-Cas9 system together with oligonucleotides composed of 40 nucleotides of upstream and downstream...

Topics

• CRISPR-Cas Systems
• Ustilago
• Gene Knockout Techniques
• Mutation
• Basidiomycota