Article
A highly efficient identification of mutants generated by CRISPR/Cas9 using the non‑functional DsRed assisted selection in Aspergillus oryzae.
World journal of microbiology & biotechnology - 9 Jul 2021
Li Yuzhen, Zhang Huanxin, Fan Junxia, Chen Ziming, Chen Tianming, Zeng Bin, Zhang Zhe
Abstract excerpt
The CRISPR/Cas9 system has become a great tool for target gene knock-out in filamentous fungi. It is laborious and time-consuming that identification mutants from a large number of transformants through PCR or enzyme-cut method. Here, we first developed a CRISPR/Cas9 system in Aspergillus oryzae using AMA1-based autonomously replicating plasmid and Cas9 under the control of the Aspergillus nidulans gpdA promoter....
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