Article
Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.
Nature communications - 16 Nov 2016
Svitashev Sergei, Schwartz Christine, Lenderts Brian, Young Joshua K, Mark Cigan A
Abstract excerpt
Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR-Cas9 has quickly become the technology of choice for genome editing due to its s...
Topics
- Alleles
- Base Sequence
- Biolistics
- CRISPR-Cas Systems
- DNA, Plant
- Gene Editing
- Gene Transfer Techniques
- Genes, Plant
- Multiprotein Complexes
- Mutagenesis
- Mutation
- Mutation Rate
