Article
An Efficient Colony PCR Method for Construct Verification in the Basidiomycete Ustilago maydis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026
Künzel Pascal, Müsgens Andreas, Blank Lars M, Wierckx Nick
Abstract excerpt
Rapid advances in AI-driven annotation and automation are increasing the demand for fast and reliable screening protocols for reverse genetic approaches. Traditional methods for genomic DNA extraction from the basidiomycete Ustilago maydis are often time-consuming and require expensive reagents and hazardous chemicals, making them unsuitable for large-scale mutant screening. Existing colony PCR protocols using...
Read the complete abstract on PubMedTopics
- Polymerase Chain Reaction
- DNA, Fungal
- Ustilago
- Mutation
- Basidiomycota