Article
Highly-efficient colony PCR method for red yeasts and its application to identify mutations within two leucine auxotroph mutants.
Yeast (Chichester, England) - 1 Nov 2012
Lin Xinping, Yang Fan, Zhou Yongjin, Zhu Zhiwei, Jin Guojie, Zhang Sufang, Zhao Zongbao Kent
Abstract excerpt
Red yeasts hold great promise in the production of microbial lipids and carotenoids. Genetic study of red yeasts has attracted much attention; however, rapid amplification of genes from red yeast samples remains technically challenging. Here a highly efficient method for the preparation of genomic DNA (gDNA) template, which could be directly used for PCR, was developed. Cells from colonies or liquid cultures were...
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