Article
Assembly of nucleosomal DNA in a cell-free extract from wild-type and top1- strains of Ustilago maydis.
Molecular & general genetics : MGG - 25 Oct 1995
Dutta S, Gerhold D, Kmiec E B
Abstract excerpt
An in vitro nucleosome assembly system has been established from cell-free extracts of the fungus Ustilago maydis. The extract catalyzed DNA supercoiling in the absence of exogenously added co-factors such as ATP and MgCl2 and was inhibited by moderate concentrations (200 mM) of KCl or NaCl. DNA supercoiling occurs via the formation of nucleosomes. Similar extracts, displaying the same activity, were prepared...
Topics
- Candida albicans
- DNA Topoisomerases, Type I
- DNA, Circular
- DNA, Fungal
- DNA, Superhelical
- Electrophoresis, Polyacrylamide Gel
- Endopeptidase K
- Micrococcal Nuclease
- Mutation
- Nucleosomes
- Saccharomyces cerevisiae