A study on the directed engineering and multiple transformations of cannabidiolic acid synthase to enhance the expression level of the recombinant enzyme.

doi:10.1007/s00203-026-05097-w

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A study on the directed engineering and multiple transformations of cannabidiolic acid synthase to enhance the expression level of the recombinant enzyme.

Archives of microbiology - 29 Aug 2026

Dai Lingyan, Lin Jishun, Kang Yue, Chi Jian, Cao Ning, Zhang Yuke, Li Panjie

Abstract excerpt

To increase the activity of cannabidiolic acid synthase (CBDAS) and its expression levels in yeast, this study focused on the CBDASG183V-N482W mutant. Using computer-aided techniques and literature reviews, four mutation sites were further identified, resulting in the mutant CBDASH114E-S116A-C176Y-G183V-N328Q-N482W. The CBDAS gene was integrated into the Pichia pastoris genome via multiple transformation rounds,...

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PMID: 42667399
DOI: 10.1007/s00203-026-05097-w

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