5' Rapid Amplification of cDNA Ends (5' RACE)-Based Targeted RNA Sequencing Assay for the Analysis of Clinically Relevant Alterations in Lung Cancer Patients.

doi:10.3390/ijms27167226

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Article

5' Rapid Amplification of cDNA Ends (5' RACE)-Based Targeted RNA Sequencing Assay for the Analysis of Clinically Relevant Alterations in Lung Cancer Patients.

International journal of molecular sciences - 13 Aug 2026

Mitiushkina Natalia V, Preobrazhenskaya Elena V, Romanko Aleksandr A, Shestakova Anna D, Belova Rimma S, Velyukhova Tatiana Y, Tretyakova Yana I, Firsova Natalia A, Nalivalkina Ekaterina A, Tiurin Vladislav I, Imyanitov Evgeny N

Abstract excerpt

Next-generation sequencing (NGS) is currently regarded as a preferable method for detection of actionable alterations in non-small cell lung carcinomas (NSCLCs). RNA-based NGS is particularly efficient in detection of gene fusions and allows for mRNA expression analysis of relevant genes. This study describes a novel library preparation pipeline for targeted RNA sequencing, which is based on the 5' rapid...

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