Article
Genome shuffling enables quantitative trait locus mapping in Bacillus subtilis.
Nature communications - 5 Aug 2026
Vasileva Delyana P, Chhetri Hari B, Hochanadel Leah H, Streich Jared C, Lagergren John H, Lane Matthew J, Mhatre Apurv, Sacko Oumar, Allemann Marco N, Oksuz Edanur, Carper Dana L, Rubino Benjamin, Chitwood Sadie, Schmitz Zachary D, Klingeman Dawn M, Abraham Paul E, Giannone Richard J, Jacobson Daniel A, Michener Joshua K
Abstract excerpt
Genetic mapping is a powerful tool for eukaryotic genetics that has only been applied to bacteria in limited circumstances. Quantitative trait locus (QTL) mapping generally relies on sexual recombination to break linkages between genes, yet bacteria rarely undergo sufficient homologous recombination to generate suitable mapping populations. In this work, we used iterative biparental genome shuffling by protoplast...
Topics
- Bacillus subtilis
- Quantitative Trait Loci
- Chromosome Mapping
- Genome, Bacterial
- Phenotype
- DNA Shuffling
- Recombination, Genetic