Reshuffling of the Bacillus subtilis 168 genome by multifold inversion.

doi:10.1093/jb/mvm197

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Article

Reshuffling of the Bacillus subtilis 168 genome by multifold inversion.

Journal of biochemistry - 1 Jan 2008

Kuroki Azusa, Toda Tsutomu, Matsui Kuniko, Uotsu-Tomita Rie, Tomita Masaru, Itaya Mitsuhiro

Abstract excerpt

The genome of Bacillus subtilis 168 was modified to yield a genome vector for the cloning of DNA several Mb in size. Unlike contemporary plasmid-based vectors, this 4.2 Mb genome vector requires specific in vivo handling protocols because of its large size. Inversion mutagenesis, a method to modify local genome structure without gain or loss of genes, was applied intensively to the B. subtilis genome; this...

Topics

Bacillus subtilis
Chromosome Inversion
DNA Shuffling
Drug Resistance, Bacterial
Genome, Bacterial
Mutation
Neomycin

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Open in PubMed

Identifiers

PMID: 17965428
DOI: 10.1093/jb/mvm197

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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