Parasexual Crossings for Bulk Segregant Analysis in Aspergillus niger to Facilitate Mutant Identification Via Whole Genome Sequencing.

Abstract excerpt

The industrially important fungus Aspergillus niger is known to reproduce only asexually. The parasexual cycle of fungi can be used for crossing two different strains to produce segregants or progeny with combined mutations even in fungi without a known sexual cycle. In A. niger, the parasexual cycle has been extensively used to establish linkage groups and to generate genetic maps. With the advent of whole...

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