Long-read low-pass sequencing enhances variant detection in a peanut MAGIC population.

Abstract excerpt

Accurate genotyping accelerates crop improvement, yet long-read sequencing remains underused in breeding due to cost. We present a scalable long-read low-pass (LRLP) sequencing framework for high-throughput variant discovery and trait mapping. Using PacBio HiFi reads in an allotetraploid peanut (Arachis hypogaea; AABB, 2n = 4x = 40) MAGIC population, we generated both LRLP and short-read low-pass (SRLP) data. At...

Topics

• Arachis
• Genome, Plant
• Polymorphism, Single Nucleotide
• High-Throughput Nucleotide Sequencing
• Genomics
• Genetic Variation