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Analytical validation of a modified wild-type blocker qpcr assay for the sensitive DNA-based detection of NPM1 type A in acute myeloid leukemia.

Molecular biology reports - 3 Aug 2026

Hamedi-Asl Pejman, Hamedi-Asl Dariush, Rostami Shaharbano, Barati Mahmood, Amini Ali, Jafari Davod, Damerchiloo Fatemeh, Manafi Rima, Safa Majid

Abstract excerpt

BACKGROUND: This study describes the development and analytical validation of a DNA-based quantitative PCR (qPCR) assay for detecting the NPM1 Type A mutation (NPM1-A mut) in acute myeloid leukemia (AML). The assay employs a modified wild-type blocker (WTB) to preferentially suppress amplification of wild-type (WT) alleles, thereby enriching mutant targets. METHODS AND RESULTS: The modified WTB was synthesized as...

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Share to DNA Mutational Analysis
Share to Leukemia, Myeloid, Acute

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Limit of Detection

Share to Nuclear Proteins

Share to Nucleophosmin

Share to Real-Time Polymerase Chain Reaction

Share to Reproducibility of Results

Share to Sensitivity and Specificity

Source

Identifiers

PMID: 42545548
DOI: 10.1007/s11033-026-12471-w

Genes

NPM1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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