Article
A real-time polymerase chain reaction assay for rapid, sensitive, and specific quantification of the JAK2V617F mutation using a locked nucleic acid-modified oligonucleotide.
The Journal of molecular diagnostics : JMD - 1 Jul 2010
Denys Barbara, El Housni Hakim, Nollet Friedel, Verhasselt Bruno, Philippé Jan
Abstract excerpt
The JAK2V617F mutation has emerged as an essential molecular determinant of myeloproliferative neoplasms (MPNs). The aim of this study was to evaluate the analytical and clinical performances of a real-time PCR (qPCR) assay using a combination of hydrolysis probes and a wild-type blocking oligonucleotide, all containing locked nucleic acid (LNA) bases. Moreover, we validated a procedure for precise quantification...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.