Integrating Mutation-Derived and Expression Features from Single-Cell RNA Sequencing: Pitfalls of Standard Cross-Validation in Small-Cohort Settings.

Abstract excerpt

Single-cell RNA sequencing (scRNA-seq) studies increasingly combine expression-based and mutation-derived signals, but small-cohort designs with repeated runs from the same biological unit can make standard cross-validation overly optimistic. We reanalyzed PRJNA736095 (14 SRR runs from 7 GSM/donor proxies) using a GATK-centered RNA-seq variant-calling and gene-burden workflow, then evaluated mutation-derived,...

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