Article

FFixR: A Machine Learning Framework for Accurate Somatic Mutation Calling from FFPE RNA-Seq Data in Cancer

2025-10-17

Abstract excerpt

Formalin-fixed paraffin-embedded (FFPE) tissues are widely used in clinical and research settings, yet their use for detecting somatic mutations from RNA sequencing (RNA-seq) is hindered by artefactual mutations introduced by cytosine deamination and strand-specific damage. Existing FFPE noise-filtering tools are tailored to DNA-seq and rely on strand bias, rendering them unsuitable for RNA-seq. Here, we present F...

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Literature Corpus work: 40638092-4a9a-54c7-bfd7-ad3a46f9a0f5
DOI: 10.1101/2025.10.17.682574

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