Structure-guided multi-strategy engineering of 3-ketosteroid-Δ1-dehydrogenase for enhanced catalytic performance and efficient enzymatic synthesis of (11α)-11,17-dihydroxypregna-1,4-diene-3,20-dione.

Abstract excerpt

3-Ketosteroid-Δ1-dehydrogenase (KstDs, EC 1.3.99.4) is the key biocatalyst for steroid C1,2-dehydrogenation, which introduces the Δ1-double bond to significantly enhance pharmacological activity. However, the catalytic efficiency and stability of native KstDs often fall short of industrial requirements. In this study, we engineered KstD2 from Mycobacterium neoaurum DSM 1381 for the dehydrogenation of its...

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• Biocatalysis
• Directed Molecular Evolution
• Enzyme Stability
• Hydrogen-Ion Concentration