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Article

Structural basis of AtCas9 recognition of PAM mutants in underwound DNA topology.

Nature structural & molecular biology - 1 Jul 2026

Duan Min, Meng Bing, Zhou Lei, Wu Lijie, Tong Xiaohan, Huang Dongchao, Yin Hao, Liu Zhi-Jie, Zhang Ying

Abstract excerpt

The CRISPR-Cas9 system locates targets through guide RNA pairing and recognition of a protospacer-adjacent motif (PAM). Although PAM specificity is sequence-determined, DNA topology can relax PAM requirements and enable near-PAMless cleavage by the type II-C Alicyclobacillus tengchongensis Cas9 (AtCas9). However, the structural mechanism underlying this regulation remains unknown. Here we report...

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CRISPR-Associated Proteins
CRISPR-Cas Systems
Cryoelectron Microscopy
DNA
PAM gene

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Models, Molecular

Nucleic Acid Conformation

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Identifiers

PMID: 42342973
DOI: 10.1038/s41594-026-01831-6

Genes

PAM

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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