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Article

Structural basis of target DNA recognition by CRISPR-Cas12k for RNA-guided DNA transposition.

Molecular cell - 4 Nov 2021

Xiao Renjian, Wang Shukun, Han Ruijie, Li Zhuang, Gabel Clinton, Mukherjee Indranil Arun, Chang Leifu

Abstract excerpt

The type V-K CRISPR-Cas system, featured by Cas12k effector with a naturally inactivated RuvC domain and associated with Tn7-like transposon for RNA-guided DNA transposition, is a promising tool for precise DNA insertion. To reveal the mechanism underlying target DNA recognition, we determined a cryoelectron microscopy (cryo-EM) structure of Cas12k from cyanobacteria Scytonema hofmanni in complex with a single...

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Identifiers

PMID: 34450043
DOI: 10.1016/j.molcel.2021.07.043
PMCID: PMC8571069

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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