Targeted next-generation sequencing implementation in Eswatini identifies rifampicin and bedaquiline resistance undetected by routine diagnostic testing.

doi:10.1038/s41467-026-73551-w

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

Targeted next-generation sequencing implementation in Eswatini identifies rifampicin and bedaquiline resistance undetected by routine diagnostic testing.

Nature communications - 11 Jun 2026

Vambe Debrah, Kay Alexander, Ziyane Mangaliso, Thunzini Mdigo, Thi Sein Sein, de Araujo Leonardo, Dreyer Viola, Niemann Tanja, Sibandze Busizwe, Masina Sijabu, Cabibbe Andrea Maurizio, Furin Jennifer, Mazuruse Tafadzwa, Dlamini Lindokuhle, Jele Thulani, Mamba Bheki, Maphalala Gugu, Dlamini Makhosazana, Dube Mbongeni, Lukhele Nomthandazo, Cirillo Daniela Maria, Mandalakas Anna, Lange Christoph, Ngwenya Sphiwe, Utpatel Christian, Dlamini Sindisiwe, Niemann Stefan

Abstract excerpt

In Eswatini, multidrug-resistant (MDR) Mycobacterium tuberculosis (Mtb) strains harbouring the rifampicin-resistance (RR) rpoB I491F mutation are missed by routine diagnostics, including GeneXpert MTB/RIF Ultra (Xpert Ultra), line probe assays (LPA), and mycobacterium growth indicator tube (MGIT) phenotypic drug susceptibility testing (pDST). To address this diagnostic gap, Eswatini introduced targeted...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antitubercular Agents

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Diagnostic Tests, Routine

Share to Diarylquinolines

Share to DNA-Directed RNA Polymerases

Share to Drug Resistance, Multiple, Bacterial

Share to Mycobacterium tuberculosis

Share to Rifampin

Share to Tuberculosis, Multidrug-Resistant

Source

Open in PubMed Open full text

Identifiers

PMID: 42270607
DOI: 10.1038/s41467-026-73551-w
PMCID: PMC13462916

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Targeted next-generation sequencing implementation in Eswatini identifies rifampicin and bedaquiline resistance undetected by routine diagnostic testing.PMID 42270607Read ↗

Detection of I491F and V170F rpoB mutations associated with misdiagnosis of rifampicin resistance among patients with drug-susceptible tuberculosis treatment failure, Myanmar, 2022.Semantic proximity Read ↗

Differential Impact of the rpoB Mutant on Rifampin and Rifabutin Resistance Signatures of Mycobacterium tuberculosis Is Revealed Using a Whole-Genome Sequencing Assay.Semantic proximity Read ↗

Implementation of Whole Genome Sequencing of Tuberculosis Isolates in a Referral Center in Rome: Six Years’ Experience in Characterizing Drug-Resistant TB and Disease TransmissionSemantic proximity Read ↗

Direct detection of drug-resistant Mycobacterium tuberculosis using targeted next generation sequencingSemantic proximity Read ↗

Phenotypic drug resistance and genome sequencing based identified mutations linked to resistance in Mycobacterium tuberculosis isolated from extrapulmonary clinical specimens.Semantic proximity Read ↗

MDR M. tuberculosis outbreak clone in Eswatini missed by Xpert has elevated bedaquiline resistance dated to the pre-treatment era.Semantic proximity Read ↗

Targeted next-generation sequencing - a promising approach in the diagnosis of Mycobacterium tuberculosis and drug resistanceSemantic proximity Read ↗

Nanopore-based targeted sequencing test for direct tuberculosis identification, genotyping, and detection of drug resistance mutations: a side-by-side comparison of targeted next-generation sequencing technologies.Semantic proximity Read ↗

[Clinical application of targeted next generation sequencing in detecting rifampicin and rifabutin resistance in tuberculosis patients].Semantic proximity Read ↗

Evaluating the efficacy of whole genome sequencing in predicting susceptibility profiles for first-line antituberculosis drugs.Semantic proximity Read ↗

Value of routine whole genome sequencing for Mycobacterium tuberculosis drug resistance detectionSemantic proximity Read ↗

Rapid drug resistance prediction in positive Mycobacterium tuberculosis clinical samples using an extensive targeted next-generation sequencing panel.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.