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Article

Brieflow: an integrated computational pipeline for high-throughput analysis of optical pooled screening data.

Nature communications - 30 May 2026

Di Bernardo Matteo, Kern Roshan S, Cepeda Diaz Ana Karla, Mallar Alexa, Choi Samuel J, Nutter-Upham Andrew, Lourido Sebastian, Blainey Paul C, Cheeseman Iain

Abstract excerpt

Optical pooled screening (OPS) has emerged as a powerful technique for functional genomics, enabling researchers to link genetic perturbations with complex cellular morphological phenotypes at scale. However, OPS data analysis presents challenges due to massive datasets, complex multi-modal integration requirements, and the absence of standardized frameworks. Here, we present Brieflow, a computational pipeline...

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Identifiers

PMID: 42218140
DOI: 10.1038/s41467-026-73643-7
PMCID: PMC13392126

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