Characterizing and circumventing sequence restrictions for synthesis of circular RNA in vitro.

doi:10.1093/nar/gkaa1256

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Article

Characterizing and circumventing sequence restrictions for synthesis of circular RNA in vitro.

Nucleic acids research - 6 Apr 2021

Rausch Jason W, Heinz William F, Payea Matthew J, Sherpa Chringma, Gorospe Myriam, Le Grice Stuart F J

Abstract excerpt

Just as eukaryotic circular RNA (circRNA) is a product of intracellular backsplicing, custom circRNA can be synthesized in vitro using a transcription template in which transposed halves of a split group I intron flank the sequence of the RNA to be circularized. Such permuted intron-exon (PIE) constructs have been used to produce circRNA versions of ribozymes, mimics of viral RNA motifs, a streptavidin aptamer,...

Topics

Base Sequence
Exons
Humans
Introns
Mutagenesis
Mutation
Nucleic Acid Conformation
RNA, Circular

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Identifiers

PMID: 33406226
DOI: 10.1093/nar/gkaa1256
PMCID: PMC8034654

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