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Investigating the detection of two-person DNA mixtures using tri-allelic InDels.

Forensic science international. Genetics - 1 Jun 2026

Li Zhimin, Chen Yinuo, Ji Qiqi, Dou Runting, Wang Zhimin, Shao Chengchen, Xu Hongmei, Xie Jianhui

Abstract excerpt

The analysis of complex DNA mixtures, such as unbalanced or degraded mixtures, remains a challenge in current forensic practice. InDel markers yield short amplicons and do not suffer from replication slippage in PCR amplification based on small-sized insertion or deletion fragments. In this study, the performance of tri-allelic InDels in analyzing two-person mixtures was evaluated. A total of 38 tri-allelic...

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PMID: 41864095
DOI: 10.1016/j.fsigen.2026.103491

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