Article
Mistranslation from an endogenous tRNA variant in human pan-genome cell lines.
Nucleic acids research - 24 Feb 2026
Rozik Peter, Moore Henry, Lant Jeremy T, Hoffman Kyle S, Schultz Sarah K, Afzal Baasil, Chan Patricia P, Flynn Lauren E, Heinemann Ilka U, Lowe Todd M, O'Donoghue Patrick
Abstract excerpt
Human transfer RNA (tRNA) anticodon variants are a source of translation error. The tRNASerAGA-2-3 variant (G35A) occurs in 2% of the human population and causes mis-incorporation of serine at phenylalanine codons. Here, we developed a dual fluorescent reporter to quantify mis-incorporation levels in live human and murine cells and validated mistranslation by mass spectrometry. In β-lymphocytes from individuals...
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