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Tailored high-cell-density fed-batch cultivation and induction strategies for optimized production of a low-immunogenic L-asparaginase mutant in Escherichia coli.

Enzyme and microbial technology - 1 Jun 2026

Brumano Larissa Pereira, Grumbach Diego, da Silva Chaves Flaviana, Monteiro Gisele, Pessoa Adalberto, Mussatto Solange I

Abstract excerpt

An integrated upstream process was developed for the high-level production of a low-immunogenic L-asparaginase mutant (EcaII P40S/S206C) in Escherichia coli, combining high-cell-density fed-batch cultivation with controlled induction and post-induction nutrient feeding. Biomass accumulation was achieved by exponential substrate feeding, followed by pH-stat-based nutrient supply during the expression phase....

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PMID: 41780212
DOI: 10.1016/j.enzmictec.2026.110836

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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