Continuous directed evolution of isoflavone synthase to mitigate feedback inhibition: combine use of a novel developed bacteria-based biosensor and high-throughput droplet sorting.

Abstract excerpt

Genistein, a bioactive isoflavone with therapeutic potential in treating oxidative stress, cardiovascular diseases, and cancer, faces production limitations during microbial biosynthesis due to product feedback inhibition of isoflavone synthase (IFS). To overcome this bottleneck, a biosensor-assisted continuous directed evolution platform was developed. Specifically, a genistein-specific biosensor was engineered...

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