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Enhancing lipid production in plant cells through automated high-throughput genome engineering and phenotyping.

The Plant cell - 13 Feb 2025

Dong Jia, Croslow Seth W, Lane Stephan T, Castro Daniel C, Blanford Jantana, Zhou Shuaizhen, Park Kiyoul, Burgess Steven, Root Mike, Cahoon Edgar B, Shanklin John, Sweedler Jonathan V, Zhao Huimin, Hudson Matthew E

Abstract excerpt

Plant bioengineering is a time-consuming and labor-intensive process with no guarantee of achieving desired traits. Here, we present a fast, automated, scalable, high-throughput pipeline for plant bioengineering (FAST-PB) in maize (Zea mays) and Nicotiana benthamiana. FAST-PB enables genome editing and product characterization by integrating automated biofoundry engineering of callus and protoplast cells with...

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PMID: 39899469
DOI: 10.1093/plcell/koaf026
PMCID: PMC11850301

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