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Article

Structure-guided engineering of CYP82D213 for enhanced triptonide biosynthesis.

Enzyme and microbial technology - 1 May 2026

Yuan Shijun, Feng Lingfang, Xu Yao, Xia Meng, Su Ping, Hu Yating, Zhang Yifeng, Huang Luqi

Abstract excerpt

Triptonide (TN) is a highly oxidized, bioactive diterpenoid triepoxide from Tripterygium wilfordii with promising pharmaceutical applications. While the early steps of its biosynthesis have been elucidated, the mechanism responsible for the critical terminal tri-epoxidation remains to be elucidated. Here, to address the limitation posed by the low intrinsic activity of CYP82D213, we employed a comprehensive...

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PMID: 41713248
DOI: 10.1016/j.enzmictec.2026.110832

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