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Article

Computational optimization of DEK1 calpain domain solubility through integrated structural modelling and data-driven targeted mutagenesis.

Scientific reports - 8 Feb 2026

Dabiri Mohammad, Levarski Zdenko, Struhárňanská Eva, Demko Viktor, Beneš Vladimir, Turňa Jan, Stuchlík Stanislav

Abstract excerpt

The DEFECTIVE KERNEL 1 (DEK1) protein plays essential functions throughout plant development. DEK1 is a multidomain 240 kDa protein with yet unsolved 3D structure. To facilitate structural and functional studies of DEK1, here we investigate its calpain protease core domain (CysPc) from Physcomitrium patens. Using integrated structural modelling we propose targeted mutagenesis of CysPc to enhance its solubility...

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Identifiers

PMID: 41656389
DOI: 10.1038/s41598-026-38805-z
PMCID: PMC12948991

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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