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Precision detection of rifampicin-resistant rpoB_L378R mutation in Mycobacterium tuberculosis with CRISPR-Cas12a.

Analytical methods : advancing methods and applications - 19 Feb 2026

Yang Yuma, Yang Li, Ma Hao, Zhang Shuming, Zhu Yue, Zhang Sihan, Lin Xiting, La Haitao, Gu Xiumin, Ma Jing, Zhao Sihao, Yang Yinfeng, Lei Hetian, Yang Yanhui

Abstract excerpt

Rifampicin is one of the most effective anti-tuberculosis drugs. However, certain strains of Mycobacterium tuberculosis (MTB) have developed resistance to rifampicin, making it crucial to identify alternative drugs for treating rifampicin-resistant MTB infections. Mutations in the rpoB gene play a pivotal role in MTB's resistance to rifampicin. Therefore, identifying these mutations is essential for effectively...

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PMID: 41642051
DOI: 10.1039/d5ay01718a

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