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A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System

13 May 2022

Abstract excerpt

Objectives CRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens remains unclear. Methods A combined rational design and target mutation-anchoring CRISPR RNA (crRNA) screening strategy was proposed. Results A set of crRNAs was...

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PMID: 35633684
DOI: 10.3389/fmicb.2022.847373

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