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Simultaneous molecular detection of Mycobacterium tuberculosis and multidrug resistance using CRISPR-AaCas12b-based nucleic acid assay.

Frontiers in cellular and infection microbiology - 1 Jan 2026

Hu Yujiao, Zhao Dan, Diao Yanjun, Bai Congxia, Zhou Ke, Huang Fang, Li Rui, Hao Xiaoyan, Liu Hao, Liu Jiayun, Zhou Lei

Abstract excerpt

Objectives: To address the unmet need for rapid, accurate diagnosis of Mycobacterium tuberculosis (MTB) and multidrug-resistant tuberculosis (MDR-TB), we developed and validated a clustered regularly interspaced short palindromic repeats-associated protein (CRISPR-Cas)-based diagnostic assay. Methods: A multiplex-recombinase polymerase amplification (RPA) coupled CRISPR-Alicyclobacillus acidiphilus Cas12b...

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Identifiers

PMID: 42239534
DOI: 10.3389/fcimb.2026.1844184
PMCID: PMC13226185

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