Article
Application of engineered CRISPR/Cas12a variants with altered protospacer adjacent motif specificities for the detection of isoniazid resistance mutations in Mycobacterium tuberculosis.
Microbiology spectrum - 7 Oct 2025
Liu Peng, Zhang Jinping, Gong Yaqi, Liu Wenqi, Xiao Guohui, Liang Juan, Wang Xuan, Bi Jing, Zhang Guoliang
Abstract excerpt
Drug-resistant tuberculosis (TB) is a major global public health concern. Although isoniazid is currently considered one of the most effective first-line drugs for TB treatment, its efficacy is limited by the emergence of resistance. Therefore, it is imperative to develop new methods for detecting drug-resistant TB. In this study, we developed a nucleic acid detection system based on the clustered regularly...
Read the complete abstract on PubMedTopics
- Isoniazid
- Mycobacterium tuberculosis
- CRISPR-Cas Systems
- Antitubercular Agents
- Bacterial Proteins