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Deciphering functional redundancy of lysine decarboxylases in probiotic E. coli Nissle 1917 via an integrated CRISPR-FTIR phenomics platform.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy - 15 Apr 2026

Zhang Yiqing, Wu Mingyu, Geng Feng, Wang Yadi, Lü Junhong

Abstract excerpt

Fourier transform infrared (FTIR) microspectroscopy is a rapid, label-free tool for microbial metabolic phenotyping. Here, we integrate synchrotron-based FTIR microspectroscopy with CRISPR-Cas9 editing to decipher the functional redundancy of lysine decarboxylases (LdcC1 and LdcC2) in probiotic Escherichia coli Nissle 1917. Under lysine stress, isogenic mutants (ΔldcC1, ΔldcC1ΔldcC2) exhibited distinct FTIR...

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PMID: 41579807
DOI: 10.1016/j.saa.2026.127508

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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