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Article

Facile solid-liquid biphasic PCR platform for ultrasensitive multiplex detection and genotyping of HPV.

Mikrochimica acta - 16 Dec 2025

Lu Zhuoxuan, Zeng Wentao, Liu Shengnan, Cao Rong, Nilghaz Azadeh, Zhang Liming

Abstract excerpt

An innovative multiplex molecular detection method is introduced, ingeniously combining solid-phase PCR with liquid-phase PCR through the assistance of magnetic fluorescent-coded microspheres. In the amplification system, the liquid-phase PCR continuously generates target DNA fragments. Using these target DNA fragments as templates, specific primers on corresponding magnetic fluorescent-coded microspheres...

Read the complete abstract on PubMed

Topics

Multiplex Polymerase Chain Reaction
Humans
Limit of Detection
DNA, Viral
Papillomaviridae
Genotype

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Genotyping Techniques

Female

Microspheres

Fluorescent Dyes

Papillomavirus Infections

Source

Identifiers

PMID: 41402514
DOI: 10.1007/s00604-025-07678-w

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 24 Oct 2026.

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